The Genomics Unit provides expertise in genomics and sequence analysis and develops new services in these fields at the request of core users.

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Genomics Unit Personnel

David Looney, MD
Genomics Unit Lead

Pinyi Du
Research Associate

Harrold Amogan
Research Associate

Genomics Unit Services

• DNA isolation
• RNA isolation
• cDNA synthesis
• Nucleic acid quantification NanoDrop One
• Nucleic acid quantification Qubit 2.0 fluorometer
• Nucleic acid quality control Agilent Bioanalyzer 2100 / TapeStation
• Nucleic acid quality control Agilent Bioanalyzer 2200 / TapeStation
• RT-qPCR full service
• RT-qPCR run
• ddPCR full service
• ddPCR run
• Gene expression quantification consultation
• Primer / probe design
• SNP genotyping
• 10X Chromium Controller use
• 10X sample and library preparation
• Targeted sequencing (AmpliSeq)
• Targeted genome sequencing
• RNA-Seq consultation
• Data preparation for downstream analysis
• Bioinformatics training

The Genomics Unit is equipped with the following instruments:

Nucleic acid characterization:

• NanoDrop One, shared access to Qubit 2.0 Fluorometer
• Agilent Bioanalyzer 2100,  shared access to Tape Station 2200
• 2 Bio-Rad T100 dedicated thermocyclers, shared access to Veriti thermocyclers
• Applied Biosystems 7500 and 7900HT Real-time PCR systems (with multiple block types)
• QuantStudio Flex7 system
• Bio-Rad (formerly QuantaLife) QX200 digital PCR instrument

Next generation sequencing:

• Ion S5/Ion Chef Sequencer
• Illumina MiSeq Desktop Sequencer
• 10X Genomics Chromium Controller
• PowerEdge T620 Tower Server (Ubuntu Linux)

Location & Contact Information

Stein Clinical Research Building, 3rd Floor Room 308
UC San Diego
9500 Gilman Drive, MC 0679
La Jolla, CA 92093-0679
Phone: 858-552-8585, ext. 2585
Email: cfargenomics@ucsd.edu