The Genomics Unit provides expertise in genomics and sequence analysis and develops new services in these fields at the request of core users.

Genomics Unit Personnel

David Looney, MD
Genomics Unit Lead

Pinyi Du
Research Associate

Harrold Amogan
Research Associate

Genomics Unit Services

• DNA isolation
• RNA isolation
• cDNA synthesis
• Nucleic acid quantification NanoDrop One
• Nucleic acid quantification Qubit 2.0 fluorometer
• Nucleic acid quality control Agilent Bioanalyzer 2100 / TapeStation
• Nucleic acid quality control Agilent Bioanalyzer 2200 / TapeStation
• RT-qPCR full service
• RT-qPCR run
• ddPCR full service
• ddPCR run
• Gene expression quantification consultation
• Primer / probe design
• SNP genotyping
• 10X Chromium Controller use
• 10X sample and library preparation
• Targeted sequencing (AmpliSeq)
• Targeted genome sequencing
• RNA-Seq consultation
• Data preparation for downstream analysis
• Bioinformatics training

The Genomics Unit is equipped with the following instruments:

Nucleic acid characterization:

• NanoDrop One, shared access to Qubit 2.0 Fluorometer
• Agilent Bioanalyzer 2100,  shared access to Tape Station 2200
• 2 Bio-Rad T100 dedicated thermocyclers, shared access to Veriti thermocyclers
• Applied Biosystems 7500 and 7900HT Real-time PCR systems (with multiple block types)
• QuantStudio Flex7 system
• Bio-Rad (formerly QuantaLife) QX200 digital PCR instrument

Next generation sequencing:

• Ion S5/Ion Chef Sequencer
• Illumina MiSeq Desktop Sequencer
• 10X Genomics Chromium Controller
• PowerEdge T620 Tower Server (Ubuntu Linux)

Location & Contact Information

Stein Clinical Research Building, 3rd Floor Room 308
UC San Diego
9500 Gilman Drive, MC 0679
La Jolla, CA 92093-0679
Phone: 858-552-8585, ext. 2585
Email: cfargenomics@ucsd.edu