Welcome to the CFAR Protein Expression and Proteomics Core

 

Co-Directors:

Bruce E. Torbett, PhD Associate Professor, Scripps Research Institute Molecular and Experimental Medicine and Immunology Email: betorbet@scripps.edu  Phone: (858) 784-9123	John H. Elder, PhD Professor, Scripps Research Institute Molecular Biology   Email: jelder@scripps.edu Phone: (858) 784-8270

What is the Protein Expression and Proteomics (PEP) Core?

The intent of the PEP Core is to provide full protein expression and proteomic services that are non-overlapping and complementary with the Molecular Biology Core and are seamlessly integrated with all established CFAR Cores. Review a summary of core services (PDF). The Protein Expression portion of the Core, under the direction of Dr. John Elder, will maintain and provide to the investigators the appropriate vectors and cell lines needed to facilitate expression cloning of proteins of interest and will aid in protein production. The Proteomics portion of the Core, under the direction of Dr. Bruce Torbett, will provide protein analysis services for CFAR Investigators. These services include protein extraction from viruses, cells, and tissues; protein resolution and quantification using 1- and 2-dimensional (D) electrophoresis and appropriate imaging systems; and protein identification using mass spectroscopy. We have recently purchased a Bio-Plex instrument for use by the CFAR community to offer Luminex technology for identification of up to 100 proteins or analytes from a single, small volume sample. We believe this technology complements the current protein expression, resolution, and identification capacity of the PEP Core. All facets of the Core are currently operational at The Scripps Research Institute.

Request Core Services

A username and are password are required to access Protein Expression and Proteomics Core services.
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