Core Services
Review a summary of core services (PDF). See details below.
Flow Cytometry
Multiparameter cell phenotyping: 2 to 6 color, antibody combinations.
Isolation of phenotypic cell subsets: fluorometric cell sorting; magnetic bead separation.
Analysis of cell activation and proliferation: cell cycle/DNA content using PI, 7-AAD, and/or BrdU, Tunel staining, AnnexinV expression; proliferation using CFSE dye; activation using Ca++ flux, surface expression of CD69, internal expression of Ki-67, cdK, bcl-2, or p53.
Analysis and quantitation of internal proteins: cytokines and chemokines; cell signaling regulatory proteins; HIV regulatory and structural proteins.
Quantitation of molecular promoters and reporter genes: expression of GFP, beta-gal, HSA, murine Thy-1.
Analysis of fixed-cell, slide preparations: multiparameter cell phenotyping and DNA analysis.
Large volume, high-speed cell sorting; and single-cell deposition and cloning.
Live cell sorting of biohazardous samples.
Functional Immunology / Clinical Research Assays
(For these assays, contact Dr. Celsa Spina at cspina@ucsd.edu.)
Lymphocyte proliferation assays (LPA): antigen-specific responses to HIV, CMV, HSV, HBV, M. tb, M. avium, and recall antigens (TT, Candida).
ELISpot assays: antigen-specific induction of cytokines (IFNg, IL-2, TNF) and CD40L expression.
Instrumentation
BD Biosciences FACSCanto is equipped with two lasers: solid state 488 nm and 633 HeNe. It is a fully digital instrument that is capable of 6 color analysis, with enhanced separation and detection of dim fluorescence peaks.
DakoCytomation MoFlo is equipped with three lasers: Coherent Enterprise II, containing both 488 Argon and UV, and a separate Melles Griot 633 HeNe. It is a jet-in-air high-speed cell sorter capable of event rates in excess of 25,000 e/sec, and up to 9 color combinations.
Beckman Coulter Epics Elite is equipped with two lasers: 488 Argon and 633 Helium Neon (HeNe). It is capable of 4 color analysis.
